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(A) <t>HCR</t> <t>RNA-FISH</t> image of Pck1 (magenta) and Fasn (green) with DAPI-stained nuclei (blue) in the fed state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (B) Magnified images of nuclei in box in panel (A). Arrows show transcription sites (TSs) (green arrow= Fasn , white arrow= Pck1 , individual channel images of Pck1 and Fasn are shown in and ). (C) Quantitative analysis of TSs in the fed state. Lines connect TS quantification between PC and PP within a lobule (N=21 lobules). Data are from 3 individual mice and are represented with different colors. Mean ± s.d. is indicated. P -value was calculated by paired t -test, N.S. if not indicated. 627 PC nuclei, 632 PP nuclei were analyzed, Scale bar, 20 um. (D) HCR-RNA FISH image of Pck1 (magenta) and Fasn (green) with DAPI-stained nuclei (blue) in the fasted state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (E) Magnified images of nuclei in box in panel (D). White arrows highlight Pck1 TS (individual channel images of Pck1 and Fasn are shown in and ). (F) Quantitative analysis of TS in the fasted state. Lines connect TS quantification between PC and PP within a lobule (N=21 lobules). Data are from 3 individual mice and are represented with different colors. Mean ± s.d. is indicated. P -value was calculated by paired t -test, N.S. if not indicated. 705 PC nuclei, 822 PP nuclei were analyzed, Scale bar, 20 um.
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(A) <t>HCR</t> <t>RNA-FISH</t> image of Pck1 (magenta) and Fasn (green) with DAPI-stained nuclei (blue) in the fed state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (B) Magnified images of nuclei in box in panel (A). Arrows show transcription sites (TSs) (green arrow= Fasn , white arrow= Pck1 , individual channel images of Pck1 and Fasn are shown in and ). (C) Quantitative analysis of TSs in the fed state. Lines connect TS quantification between PC and PP within a lobule (N=21 lobules). Data are from 3 individual mice and are represented with different colors. Mean ± s.d. is indicated. P -value was calculated by paired t -test, N.S. if not indicated. 627 PC nuclei, 632 PP nuclei were analyzed, Scale bar, 20 um. (D) HCR-RNA FISH image of Pck1 (magenta) and Fasn (green) with DAPI-stained nuclei (blue) in the fasted state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (E) Magnified images of nuclei in box in panel (D). White arrows highlight Pck1 TS (individual channel images of Pck1 and Fasn are shown in and ). (F) Quantitative analysis of TS in the fasted state. Lines connect TS quantification between PC and PP within a lobule (N=21 lobules). Data are from 3 individual mice and are represented with different colors. Mean ± s.d. is indicated. P -value was calculated by paired t -test, N.S. if not indicated. 705 PC nuclei, 822 PP nuclei were analyzed, Scale bar, 20 um.
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Image Search Results


(A) HCR RNA-FISH image of Pck1 (magenta) and Fasn (green) with DAPI-stained nuclei (blue) in the fed state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (B) Magnified images of nuclei in box in panel (A). Arrows show transcription sites (TSs) (green arrow= Fasn , white arrow= Pck1 , individual channel images of Pck1 and Fasn are shown in and ). (C) Quantitative analysis of TSs in the fed state. Lines connect TS quantification between PC and PP within a lobule (N=21 lobules). Data are from 3 individual mice and are represented with different colors. Mean ± s.d. is indicated. P -value was calculated by paired t -test, N.S. if not indicated. 627 PC nuclei, 632 PP nuclei were analyzed, Scale bar, 20 um. (D) HCR-RNA FISH image of Pck1 (magenta) and Fasn (green) with DAPI-stained nuclei (blue) in the fasted state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (E) Magnified images of nuclei in box in panel (D). White arrows highlight Pck1 TS (individual channel images of Pck1 and Fasn are shown in and ). (F) Quantitative analysis of TS in the fasted state. Lines connect TS quantification between PC and PP within a lobule (N=21 lobules). Data are from 3 individual mice and are represented with different colors. Mean ± s.d. is indicated. P -value was calculated by paired t -test, N.S. if not indicated. 705 PC nuclei, 822 PP nuclei were analyzed, Scale bar, 20 um.

Journal: bioRxiv

Article Title: A novel subset of hepatocytes is simultaneously gluconeogenic and de novo lipogenic in the fed state and is naturally insulin resistant

doi: 10.64898/2026.03.09.710526

Figure Lengend Snippet: (A) HCR RNA-FISH image of Pck1 (magenta) and Fasn (green) with DAPI-stained nuclei (blue) in the fed state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (B) Magnified images of nuclei in box in panel (A). Arrows show transcription sites (TSs) (green arrow= Fasn , white arrow= Pck1 , individual channel images of Pck1 and Fasn are shown in and ). (C) Quantitative analysis of TSs in the fed state. Lines connect TS quantification between PC and PP within a lobule (N=21 lobules). Data are from 3 individual mice and are represented with different colors. Mean ± s.d. is indicated. P -value was calculated by paired t -test, N.S. if not indicated. 627 PC nuclei, 632 PP nuclei were analyzed, Scale bar, 20 um. (D) HCR-RNA FISH image of Pck1 (magenta) and Fasn (green) with DAPI-stained nuclei (blue) in the fasted state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (E) Magnified images of nuclei in box in panel (D). White arrows highlight Pck1 TS (individual channel images of Pck1 and Fasn are shown in and ). (F) Quantitative analysis of TS in the fasted state. Lines connect TS quantification between PC and PP within a lobule (N=21 lobules). Data are from 3 individual mice and are represented with different colors. Mean ± s.d. is indicated. P -value was calculated by paired t -test, N.S. if not indicated. 705 PC nuclei, 822 PP nuclei were analyzed, Scale bar, 20 um.

Article Snippet: HCR RNA-FISH probes (Molecular Instruments) targeting Pck1 (B3), Fasn (B1), and Glul (B2) were hybridized overnight at 37 °C (0.4 pmol of each probe set/100 uL).

Techniques: Staining

(A) HCR RNA-FISH image of Pck1 (magenta) with DAPI-stained nuclei (blue) in the fed state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (B) HCR RNA-FISH image Fasn (green) with DAPI-stained nuclei (blue) in the fed state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (C) HCR RNA-FISH image of Pck1 (magenta) with DAPI-stained (blue) in the fasted state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (B) HCR RNA-FISH image Fasn (green) with DAPI-stained (blue) in the fed state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. Scale bar, 20 um.

Journal: bioRxiv

Article Title: A novel subset of hepatocytes is simultaneously gluconeogenic and de novo lipogenic in the fed state and is naturally insulin resistant

doi: 10.64898/2026.03.09.710526

Figure Lengend Snippet: (A) HCR RNA-FISH image of Pck1 (magenta) with DAPI-stained nuclei (blue) in the fed state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (B) HCR RNA-FISH image Fasn (green) with DAPI-stained nuclei (blue) in the fed state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (C) HCR RNA-FISH image of Pck1 (magenta) with DAPI-stained (blue) in the fasted state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. (B) HCR RNA-FISH image Fasn (green) with DAPI-stained (blue) in the fed state (representative image shown). Lobules show the central vein (CV) on the left and the portal vein (PV) on the right. Scale bar, 20 um.

Article Snippet: HCR RNA-FISH probes (Molecular Instruments) targeting Pck1 (B3), Fasn (B1), and Glul (B2) were hybridized overnight at 37 °C (0.4 pmol of each probe set/100 uL).

Techniques: Staining